Journal of The School of Marine Science and Technology,Vol.1 No.1
Effects of endocrine disrupter chemicals bisphenol A and
p-nonylphenol on marine microalga Nannochloropsis oculata ST-3
Hiroshi ISHII, Hiroshi SAITO, Nobuhiko AKIYAMA and Yukio KOBAYASHI
Abstract
In aquaculture food chains, marine microalga Nannochloropsis oculata is used as a primary productivity. Industrial
pollution of endocrine disrupter chemicals (EDs) has recently broken out in the coastal aquaculture. Effects of EDs on
marine microalga N. oculata ST-3 strain were investigated with bisphenol A (BPA) and p-nonylphenol (p-NP). The
concentration of BPA and p-NP in the algal culture was controlled at 1 (or 1.5),3,6,9 and 18ppm for examining growth
curve of ST-3 strain. The growth of ST-3 strain was not affected by both EDs up to the doses of 3ppm,but it was at the
concentration of BPA and p-NP in the range of 6-18 ppm.Furthermore,the cultures of ST-3 strain,contaminated by each
EDs at 3ppm, were compared with the culture at 0ppm on different temperature conditions;20゜C and 30゜C. These growth
rates at 30゜C in the presence of 3ppm EDs were significantly higher than those of 20゜C. At the stationary phase, the
distribution of intra-and extra-cellular EDs was investigated under two different temperatures (20゜C and 30゜C）. The
intracellular BPA increased with the decrease in extracellular BPA from 0.0131 to 0.0955 ppm at 20゜C.It showed 7.3 times
higher than that at time zero. Therefore, ST-3 strain might have bioaccumulated at the low concentration of BPA.