Journal of The School of Marine Science and Technology,Vol.4 No.3
Formaldehyde degradation capability of the aerobic bacteria
isolated from the Izu Peninsula, Shizuoka
Hiroshi ISHII, Kosuke YOSHIDA, Ryoichi INOUE, Yoshihiro YAMADA,
Hiroshi SAITO and Yoshihiro OKADA
Abstract
Formaldehyde is a highly toxic chemical for all organisms, and it has recently been found as effluent from industrial waste water to marine environment. The aims of this study were to investigate the effect of 10ppm formaldehyde on the microbial communities isolated from five coastal sea water samples, collected from the Izu Peninsula,Japan.Rapid decrease of formaldehyde concentration was only observed in a raw sample at the Kifu area of Numazu city,but no decrease of this boiled sample.Six bacterial strains were isolated from the Kifu area by using spread plate method. Effects of concentrations of 10ppm formaldehyde on each pure-cultured bacterial strain were investigated in batch cultures.During the first ‚R days,ca.95“ (v/v)of formaldehyde was rapidly decreased by three bacterial strains (BR-41, ZB-51, and T1{1). When the formaldehyde was used as sole carbon source in the M9 medium, these strains were not able to grow in the absence of glucose.However in the presence of glucose, 10ppm formaldehyde did not inhibit the growth of the strain BR-41.And rapid decrease of formaldehyde concentration was observed, indicating that the tolerance to 10ppm formaldehyde of strain BR-41 was occurred in M ‚X medium. Furthermore,in case of addition the trace metals to M ‚X medium including 10ppm formaldehyde concentration,the strain BR-41 was able to grow well and the 90“ (v/v) of formaldehyde was decreased of within 12h of the incubation. The formaldehyde-resistant strain BR-41was classified to the family Alteromonadaceae by16S rRNA gene sequence analysis.
@ @ @
@ PDFi0.5MBj >>>
@ @ @