Journal of The School of Marine Science and Technology,Vol.9 No.3
Making divergent marker of tropical and temperate types of Oryza sativa L. var. japonica based on insertion / deletion DNA region and its utilization of carbonated rice from ToroⅠsite
Thirty one varieties of tropical and temperate types of japonica from fourteen countries and twenty three varieties of indica from eleven countries, totally fifty four rice （Oryza sativa L.） varieties were used. Genetic differentiation was detected between tropical and temperate type. The differentiation is shown apparently in the divergence in which there are two hundred twenty one base pairs in a region （DJ6） insertion/deletion on the chromosome 6. In this region, temperate type showed a deletion, whereas tropical type and indica type indicated specific sequences in DJ6 region. Ten primers were designed for detection of DJ6 region. The combinations of new primers were performed in the deleted region and obviously identified tropical type. Also carbonated rice was analyzed using the set of those primers. Only seven grains of carbonated rice which were excavated from Toro I site in Yayoi era were used. The results showed two temperate types and one hybrid type including in tropical and temperate types. It is shown there were already been naturally hybridized tropical and temperate types in the Late Yayoi era.